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nsii sali sites  (Addgene inc)


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    Structured Review

    Addgene inc nsii sali sites
    Nsii Sali Sites, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nsii+sali+sites/pCMV-IRES-Renilla+Luciferase-IRES-Gateway-Firefly+Luciferase+(pIRIGF)+(Plasmid+%23101139)/pm34730395-214-28-32
    Average 93 stars, based on 8 article reviews
    nsii sali sites - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: Host Restriction Factor A3G Inhibits the Replication of Enterovirus D68 by Competitively Binding the 5' Untranslated Region with PCBP1.
    Article Snippet: RNAs of EVD68, CA6, and HEK293T cells were extracted with TRIzol (Invitrogen, Carlsbad, CA, USA) and reverse-transcripted with oligonucleotide (dT) primers and M-MLV reverse transcriptase (Invitrogen) according to the manufacturer’s instructions. .. The resulting cDNA was used for amplification of the EVD68 59 UTR, EVD68 2C, PCBP1, and PCBP2; then, an amplified fragment of 59 UTR was inserted into the NsiI/SalI sites of pIRIGF (Addgene, catalog no. 101139) or the SalI/BamHI sites of the VR1012 vector to generate the plasmid constructs 59 UTR-luciferase-pIRIGF and 59 UTR-VR1012. ..

    Article Title: Host Restriction Factor A3G Inhibits the Replication of Enterovirus D68 by Competitively Binding the 5′ Untranslated Region with PCBP1
    Article Snippet: RNAs of EVD68, CA6, and HEK293T cells were extracted with TRIzol (Invitrogen, Carlsbad, CA, USA) and reverse-transcripted with oligonucleotide (dT) primers and M-MLV reverse transcriptase (Invitrogen) according to the manufacturer’s instructions. .. The resulting cDNA was used for amplification of the EVD68 59 UTR, EVD68 2C, PCBP1, and PCBP2; then, an amplified fragment of 59 UTR was inserted into the NsiI/SalI sites of pIRIGF (Addgene, catalog no. 101139) or the SalI/BamHI sites of the VR1012 vector to generate the plasmid constructs 59 UTR-luciferase-pIRIGF and 59 UTR-VR1012. ..

    Plasmid Preparation:

    Article Title: Host Restriction Factor A3G Inhibits the Replication of Enterovirus D68 by Competitively Binding the 5' Untranslated Region with PCBP1.
    Article Snippet: RNAs of EVD68, CA6, and HEK293T cells were extracted with TRIzol (Invitrogen, Carlsbad, CA, USA) and reverse-transcripted with oligonucleotide (dT) primers and M-MLV reverse transcriptase (Invitrogen) according to the manufacturer’s instructions. .. The resulting cDNA was used for amplification of the EVD68 59 UTR, EVD68 2C, PCBP1, and PCBP2; then, an amplified fragment of 59 UTR was inserted into the NsiI/SalI sites of pIRIGF (Addgene, catalog no. 101139) or the SalI/BamHI sites of the VR1012 vector to generate the plasmid constructs 59 UTR-luciferase-pIRIGF and 59 UTR-VR1012. ..

    Article Title: Host Restriction Factor A3G Inhibits the Replication of Enterovirus D68 by Competitively Binding the 5′ Untranslated Region with PCBP1
    Article Snippet: RNAs of EVD68, CA6, and HEK293T cells were extracted with TRIzol (Invitrogen, Carlsbad, CA, USA) and reverse-transcripted with oligonucleotide (dT) primers and M-MLV reverse transcriptase (Invitrogen) according to the manufacturer’s instructions. .. The resulting cDNA was used for amplification of the EVD68 59 UTR, EVD68 2C, PCBP1, and PCBP2; then, an amplified fragment of 59 UTR was inserted into the NsiI/SalI sites of pIRIGF (Addgene, catalog no. 101139) or the SalI/BamHI sites of the VR1012 vector to generate the plasmid constructs 59 UTR-luciferase-pIRIGF and 59 UTR-VR1012. ..

    Construct:

    Article Title: Host Restriction Factor A3G Inhibits the Replication of Enterovirus D68 by Competitively Binding the 5' Untranslated Region with PCBP1.
    Article Snippet: RNAs of EVD68, CA6, and HEK293T cells were extracted with TRIzol (Invitrogen, Carlsbad, CA, USA) and reverse-transcripted with oligonucleotide (dT) primers and M-MLV reverse transcriptase (Invitrogen) according to the manufacturer’s instructions. .. The resulting cDNA was used for amplification of the EVD68 59 UTR, EVD68 2C, PCBP1, and PCBP2; then, an amplified fragment of 59 UTR was inserted into the NsiI/SalI sites of pIRIGF (Addgene, catalog no. 101139) or the SalI/BamHI sites of the VR1012 vector to generate the plasmid constructs 59 UTR-luciferase-pIRIGF and 59 UTR-VR1012. ..

    Article Title: Host Restriction Factor A3G Inhibits the Replication of Enterovirus D68 by Competitively Binding the 5′ Untranslated Region with PCBP1
    Article Snippet: RNAs of EVD68, CA6, and HEK293T cells were extracted with TRIzol (Invitrogen, Carlsbad, CA, USA) and reverse-transcripted with oligonucleotide (dT) primers and M-MLV reverse transcriptase (Invitrogen) according to the manufacturer’s instructions. .. The resulting cDNA was used for amplification of the EVD68 59 UTR, EVD68 2C, PCBP1, and PCBP2; then, an amplified fragment of 59 UTR was inserted into the NsiI/SalI sites of pIRIGF (Addgene, catalog no. 101139) or the SalI/BamHI sites of the VR1012 vector to generate the plasmid constructs 59 UTR-luciferase-pIRIGF and 59 UTR-VR1012. ..



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    93
    Addgene inc nsii sali sites
    Nsii Sali Sites, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nsii+sali+sites/pCMV-IRES-Renilla+Luciferase-IRES-Gateway-Firefly+Luciferase+(pIRIGF)+(Plasmid+%23101139)/pm34730395-214-28-32
    Average 93 stars, based on 1 article reviews
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    93/100 stars
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